A Comparative Analysis of Vitamin D3 and Biofield Energy Treated Vitamin D3 in Bone Health Using MG-63 Cell Line by Snehasis Jana* in Orthoplastic Surgery & Orthopedic Care International Journal_ Orthopedic Care International Journal
Abstract
Poor
bone health is related with various bone disease that predisposes to fractures,
while sufficient supplementation with calcium and vitamin D suggested prevention
of bone diseases. The present study aimed to find the effect of Consciousness
Energy Healing based vitamin D3 and DMEM medium on in vitro bone health
parameters using MG-63 cells such as alkaline phosphates enzyme (ALP) activity,
collagen levels, and bone mineralization. The test items (TI) i.e. vitamin D3
and DMEM medium were divided into two parts. The test samples received
Consciousness Energy Healing Treatment by Lauree Ann Duprey-Reed and samples
were defined as the Biofield Energy Treated (BT) samples, while the other parts
of each sample were denoted as the untreated test items (UT). Cell viability
using MTT assay showed that cell viability range 76% to 139% with safe and
nontoxic profile of test samples on MG-63 cell line. ALP was significantly
increased by 171.8% and 189.7% at 10μg/mL in the UT-DMEM+BT-TI and
BT-DMEM+BT-TI groups, respectively as compared with the untreated group.
In
addition, 100% and 33.3% increased ALP at 0.1 and 10μg/mL, respectively in
BT-DMEM+UT-TI group as compared with the untreated group. The level of collagen
was significantly increased at 10μg/mL by 145.8% in the UT-DMEM+BT-TI group, while 65.8%
and 145.8% at 1 and 10μg/mL, respectively in BT-DMEM+UT-TI group as compared
with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant
increased collagen level by 86.3% and 136.1% at 1 and 10μg/mL, respectively as
compared with the untreated test item and DMEM group.
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