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Thursday, November 19, 2020

A Comparative Analysis of Vitamin D3 and Biofield Energy Treated Vitamin D3 in Bone Health Using MG-63 Cell Line_ Crimson Publishers

 A Comparative Analysis of Vitamin D3 and Biofield Energy Treated Vitamin D3 in Bone Health Using MG-63 Cell Line by Snehasis Jana* in Orthoplastic Surgery & Orthopedic Care International Journal_ Orthopedic Care International Journal

 

Abstract

Poor bone health is related with various bone disease that predisposes to fractures, while sufficient supplementation with calcium and vitamin D suggested prevention of bone diseases. The present study aimed to find the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on in vitro bone health parameters using MG-63 cells such as alkaline phosphates enzyme (ALP) activity, collagen levels, and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lauree Ann Duprey-Reed and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability range 76% to 139% with safe and nontoxic profile of test samples on MG-63 cell line. ALP was significantly increased by 171.8% and 189.7% at 10μg/mL in the UT-DMEM+BT-TI and BT-DMEM+BT-TI groups, respectively as compared with the untreated group.

In addition, 100% and 33.3% increased ALP at 0.1 and 10μg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. The level of collagen was significantly increased at 10μg/mL by 145.8% in the UT-DMEM+BT-TI group, while 65.8% and 145.8% at 1 and 10μg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 86.3% and 136.1% at 1 and 10μg/mL, respectively as compared with the untreated test item and DMEM group.

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