Extraction, Purification and IndustrialApplications of Pectinase: A Review by Nawaz A in Journal of Biotechnology& Bioresearch
Pectin is a hetero-polysaccharide molecule with
1,4-linked α-D- galacturonic acid as principle component. Pectinase enzyme is
responsible for the breakage of glyosidic bonds of galacturonic acid’s residue
present in pectic substances. For the production of pectinase enzyme both types
of submerged and solid-state fermentation (SSF) are usually used. Mostly, the
production of enzyme is found better in SSF. There are certain different
techniques like filtration, dialysis, ammonium salt precipitation, gel
filtration chromatography, Ion exchange chromatography and SDS-PAGE which can
be applied for purification of pectinase enzyme. Pectinase plays an important
role in industrial processes such as fruit juice extraction, degumming of plant
fiber, waste water treatment, oil extraction, coffee and tea fermentation,
paper and pulp industry.
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