Enhanced Succinic AcidProduction in Escherichiacoli by Model-Guided Metabolic Gene Knockout of pflAUsing Glucose Carbon Source
by Bashir Sajo Mienda in Journal of Biotechnology& Bioresearch
Succinic acid is an important platform and/or
commodity or specialty chemical with a broad range of applications. The
metabolic role of pyruvate formatelyase A (pflA) in relation to succinate
production inEscherichia coli under anaerobic conditions from glucose substrate
remained largely unspecified. Herein we identified pflAgene for the first time,
as a novel gene knockout target for increasing succinate production inE. coli.
Guided by E. coli reconstruction iJO1366, we engineered the E. coli host
metabolism by deleting the pflA, thereby causing the up-regulation of
glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which hypothetically
increases the generation of NADH and the pool of phosphoenolpyruvate (PEP) in
the central carbon metabolism, required for succinate production. This strategy
produced succinic acid that is 4.78 fold (0.28g l-1 in 1day) from glucose
substrate. This work elucidatesfor the first time that pflA is a novel gene
deletion target for increasing succinic acid production in E. coli under
anaerobic conditions. In addition, these results highlight the power of
metabolic model in identifying novel gene deletion target and ultimately
driving novel biological discovery.
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